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We also retained mutants without the occurrence of root swelling after exposure to Al.
In conclusion, pHJOFpac is an ideal construct for the rapid production of VZV mutants without the retention of any unwanted operational sequences in the manipulated viral genome.
dla mutants do not have defects in Zn5+ cell number, but have similar aberrant patterning as in srn mutants, without the loss of the dorsal projecting nerve (Fig. 4A, B, dashed blue bracket and solid pink oval, respectively).
We furthermore surveyed if sequencing of only the mutants without the WT would have been already sufficient to identify the specific variants in each mutant.
K+ is certainly involved in carbohydrate translocation, as Arabidopsis mutants without the potassium channel AKT2/3 show impaired loading and transport of phloem sucrose, probably due to an effect on membrane potential (Deeken et al., 2002).
Mutants without the gene for Hst3 develop seven times as many green sectors as wild-type yeast, but fail to register derepression in conventional assays (Yang et al., 2008).
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When the glucose was used up, the strains continued to grow on the supplemented D-xylose, allowing us to select inhibitor tolerant mutants without losing the capacity to grow on D-xylose.
We reasoned that if FLP-7 and NPR-22 are a true ligand-receptor pair in vivo, neither gene could rescue the double mutant without the presence of the other.
Moreover, creation of a water channel to the heme center significantly enhanced the NIR activity compared to the corresponding mutant without the channel.
While the fusion protein localizes to the nucleus, its deletion mutant without the PHD domain resides exclusively in the nucleolus, suggesting a potential link between chromatin-binding PHD domain and nuclear architecture.
An enzymatic dead mutant without the N-terminus also has tumor suppression activity (Nesiel-Nuttman et al., 2015).
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