Sentence examples for mutants with single from inspiring English sources

We found that simultaneous mutations at three positions (E67S/E74S/E75S) of NS2/NEP were important for inhibition of influenza viral polymerase activity, although the W78S mutant and other glutamate mutants with single substitutions were not.

(A) The phosphorylated threonine and serine residues shown in Fig. 1D were mutated to alanine, and Atg31 mutants with single mutations or multiple mutations in various combinations, as well as Atg31 deletion mutants, were assessed for autophagy activity under nitrogen starvation conditions.

Consequently, SInv and 5 mutants including three mutants with single point substitution [Mut1 = P152 V, Mut2 = S85 V and Mut3 = K153F ], one mutant with two points [Mut4 = S305V-N463 V] and one mutant with three points [Mut5 = S85V-K153F-T271 V] were developed via site-directed mutagenesis and produced using Pichia pastoris as the host.

Similar differences in inhibitor efficacy were noted in bc1 complexes from yeast mutants with single amino acid substitutions at the center N site, although the binding affinity of quinone and quinol substrates were not perturbed to a degree that impaired catalytic function in the variant enzymes.

The GABA-T gene was sequenced from ten plants that phenotypically resembled pop2 mutants, and two new pop2 mutants with single point mutations were identified designated pop2-6 and pop2-7 (Figure 3A).

There was not much difference in the degrees of sensitivity among the mutants with single gene deletions or the whole operon deletion, thus suggesting their role in a common pathway.

We found both nonsense alleles leading to severe protein truncations and mutants with single-amino acid substitutions that are informative at a molecular level.

Moreover, in all three cases, the synthetic growth defect on dissection plates was stronger with esl1 Δ esl2 Δ double mutants compared with single mutants, indicative of functional redundancy between Esl1 and Esl2.

As judged by colony size and density of double mutants compared with single mutants in serial dilution assays, we detected strong negative interactions with csn1, ddb1, and pnk1; moderate negative genetic interactions with csn5, rad26, rad17, srs2, swi3, and sde2; and only weak genetic interactions with raf1 and snt1.

Little, or no, rcDNA was detected for each mutant with single alanine substitutions (Fig. 2; lanes 2,4,6).

You do find things like axes but, while they will dispatch mutants with a single blow, they disintegrate after a single use.