The four missense mutants with predicted possible damaging outcomes showed no changes in intracellular localization.
The ssrA gene in the plasmid p16Dum-Cat [ 11] was altered to express tmRNA mutants with every possible -1 triplet.
These truncated mRNAs are unstable and get degraded by specific pathways, that is, nonsense-mediated mRNA decay, which are in-built protective cellular mechanisms against mutant proteins with possible dominant negative effect [ 42, 46, 47].
However, the generation of test sets to kill all the mutants with a reduced, possible minimum, number of products is a complex task.
In order to test the LFCH, one has to construct as many mutants as possible with the following properties: 1.
The average λ for different sequence length was obtained through comparing the secondary structure of randomly created RNA sequences with that of all possible mutants with only one substitution.
The major difficulty that is faced in extracting ⟨ν⟩∞ by the method of those three studies [ 3, 27, 28] is that it is not possible to directly assay mutants with m mutations, but instead only possible to assay a set of mutants with a distribution of m.
Thus, it is possible that mutants with relatively smaller effects will be false negatives.
This means that growth of the RubisCO mutant is possible with acetate because of the lower burden of excess reductant when assimilating this substrate.
In the presence of lexA imuB dnaE2, it is possible to obtain mutants with various keratinolytic activities after UV exposure.
