Sentence examples for mutants with only from inspiring English sources

Finally, the tethered factors may create chromatin that is more accessible to mismatch repair, so a significant fraction of mutations could in principle be reverted, to create a large class of mutants with only single base changes.

The mutants with only individual cysteine residues retained weak or strong oxidation, depending on the cysteine residue, with C119 and C162 oxidized to an extent greater than residues C211 and C39.

We performed in situ hybridisation with emx2 and foxg1 riboprobes to label telencephalic structures and found that these territories were severely affected in the mutants, with only a small anterior territory being emx2 and foxg1 positive (Fig. 1E H).

By contrast, HSQC spectral dispersions are markedly abolished for all four insertion mutants, with only ∼0.85 ppm over 1H and ∼18 ppm over 15N dimensions (Figure 3b 3e) [30], [30].

To that purpose, another group recently reported development of a phage Mu-based, random transposition mutagenesis system for C. perfringens [15], which proved relatively efficient and produced mutants with only a single transposon insertion.

Since the long-term aim is to investigate the consequences of DNA damage in sperm on early embryonic development we chose mutants with only mild to moderate defects in spermatogenesis.

A constitutively dephosphorylated (S61A) tropomyosin mutant blocks agonist-dependent betaAR internalization, whereas a tropomyosin mutant that mimics constitutive phosphorylation (S61D) complements the PI(3)K mutant, with only lipid phosphorylation activity reversing the defective betaAR internalization.

Ubiquitin and ubiquitin-mutants with only Lys48 or Lys63 available for polymerization were generated via PCR and cloned in pcDNA3.1 with an N-terminal HA-tag.

To assess a possible effect of EGFP on the RING E3 ubiquitin ligase activity of TRAF6 or TRAF2, we monitored ubiquitination via a HA-tagged ubiquitin-mutant with only Lys63 available for polymerization (HA-Ub-K63).

The extracellular CD fraction was also lower in SCC-VII cells expressing either M6P/IGF2R mutant with only one functional M6P-binding site (16 43%).

The first uses a Cas9 mutant with only a single-strand endonuclease activity such as the D10A mutant that cleaves only the strand complementary with the gRNA (Cho et al., 2014; Esvelt et al., 2013; Ran et al., 2013a).