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Indeed mutants with major improvements in k cat may display only minor changes in active site structure.
In their new paper, Diana Blank and collaborators have taken a similar approach by experimentally evolving E. coli mutants with major gene deletions to test if and how the mutants are able to regain function (Blank et al. 2014).
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Previous results [8] detect no more than two parameters from the hmg1 and hmg2 mutants with normal distributions on one side at P<0.0001, suggesting that our high-content image-profiling method can detect mutants of genes with major cellular roles even if the mutant show a weak phenotype.
Gars 201R/201R homozygous mutants, however, only survive for a few weeks with major defects in the CNS observed.
These data raise the possibility that mutant miR182 contributes to circadian clock ailments associated with major depression [ 82].
Struggling with major depression?
Seek job with major company.
Start with major maternity retailers.
In contrast to what was observed for dasatinib binding to wild type Abl, when dasatinib was incubated at the same molar ratio with the T315I mutant, no major changes in HX were detected.
A major problem for existing anti-retroviral therapy is the emergence of drug-resistant mutants with reduced susceptibility to the inhibitors.
Mutants with S/− or S*/− phenotypes showed positive biological behaviour with respect to virus multiplication and therefore probably did not reflect major defects in protein folding.
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