In this study, the iterative ARTP mutagenesis was applied to S. mobaraensis for breeding mutants with increased TGase production.
Because of its remarkable reiterative design, the fly eye lends itself to studies of mutants with increased or decreased apoptosis.
The top eight mutants with increased TGase production were chosen as the starting strains for the next round of ARTP mutagenesis.
Vice versa, engineered mutants with increased cooperativity show enhanced binding to proapoptotic genes, which shifts the cellular response to cell death.
To confer effector functions and simultaneously increase the potency, we first matured m36 by panning and screening a mutant library for mutants with increased binding to gp120.
To improve the fermentation production of transglutaminase (TGase) from Streptomyces mobaraensis for applications in the food industry, the atmospheric and room-temperature plasma (ARTP) mutagenesis was applied to breed S. mobaraensis mutants with increased TGase production.
Moreover, all process variables influencing the production yield, different simple and complex carbon sources, the metabolic pathway of production, engineered mutants with increased productivity, and modified tolerance against high concentrations of acid have been described.
In this issue of Structure, Rincón and colleagues show that electrostatic repulsion within the capsid is responsible for this lability, and they present rationally designed mutants with increased thermostability.
All mutants with increased basal activity were close to the EL1/TM2 or EL1/TM3 boundaries.
Recently Dötsch et al. [18] screened a transposon mutant library of PA14 for mutants with increased resistance or susceptibility.
