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We designed and proposed two high-throughput screening schemes for selecting l-threonine aldolase mutants with improved properties.
Here, we present a method that identifies protein mutants with improved overall cellular efficacy, an objective not feasible with previous approaches.
This approach allows the selection of mutants with improved stability, a parameter of great interest in any biotechnological application of photosynthetic proteins spanning from space research to biosensoric and photovoltaic fields, including biofuel and nutraceuticals production [44].
Computational strain design methods evaluate the effects of gene or reaction deletions to search for the mutants with improved production capabilities.
The use of recombinant HRP mutants with improved stability behavior (e.g. Morawski et al. 2001; Ryan and O'Fágáin 2007a), as well as upcoming studies thereon are likely to yield further beneficial contributions in the near future.
This approach provides an alternative route for identifying transcription factor mutants with improved tolerance against various stresses, and will be an effective strategy for improving other similar complex phenotypes involved in multiple genes in the future.
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The engineering of enzymes by rational design or directed evolution uses different methodologies to optimize existing enzymes by construction of mutant libraries followed by their testing to identify mutant proteins with improved properties.
This mini-review summarizes the recent advances in development of mutant luciferases with improved stability and activity characteristics.
Using this strategy, scFv mutants with 8 9 °C improved thermal stability and 0.8 0.9 M improved stability for guanidinium chloride were found after 4 5 enrichment cycles.
The conservation of residues important for thermostability and catalytic ability enabled us to fast design, construct, and test the mutants with substantially improved characters in breadmaking.
The carotenoid biosynthesis inhibitor (and herbicide) diphenylamine was used to screen for mutants with potentially improved carotenogenic capacity.
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