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We obtained several mutants with higher activity, del1 2, del1 3, and S2R.
A truncated version and full-length mutants with higher affinity than the parent sequence are shown.
Mutants with higher kon and koff rates gave rise to higher basal signaling and gene expression but impaired osmoadaptation.
Through multiple selection cycles, in presence of increasing concentrations of the selection pressure, i.e. the toxic compound, mutants with higher tolerance can be selected.
Therefore, the double mutations T166A/E539K and K312E/E539K were designed in the hope of obtaining mutants with higher catalytic efficiency and better thermostability.
The identified mutants with higher oxidative tolerance and catalytic efficiencies would be helpful for further improving the oxidative stability of versatile peroxidase.
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Many interesting mutants with high activity were identified from the metagenome of bacteria in chemical plant sludge.
Physical and chemical mutagens have been used in mutation breeding and mutants with high Zn have been identified.
First, an in vitro CYP BM3 mutant library screen was performed to identify mutants with high activity, as well asstereoselectivity.
This phenotype may ease the emergence of mutants with high-level fluoroquinolone resistance under fluoroquinolone monotherapy, especially when the bacterial inoculum is high [85].
In the present study, to efficiently isolate mutants with high-level organic solvent tolerance, the parent strain JA300 was precultured in the LBGMg liquid medium overlaid with cyclohexane.
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