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Although DTR mutants with extended JMs showed normal DT binding activity, the cells expressing the mutants showed both reduced translocation of DT fragment A into the cytosol and reduced sensitivity to DT, when compared with cells expressing wild-type DTR.
10.7554/eLife.07403.008 Figure 5. Assisting load can rescue the velocity of mutants with extended NLs.
Similarly, yeast mutants with extended lifespan were also identified (Table 4), and a significant extension of lifespan was obtained for the mutants ΔCYS4, ΔALD4, and ΔPDX3 (Fig. 3B).
Nevertheless, regardless of species or insert sequence, none of the mutants with extended NL domains that retained their native cysteine residues exhibited either processive backstepping under superstall loads or velocities surpassing the WT under assisting loads.
5) In the subsection headed "The force-dependence of kinesin mutants with extended neck linkers can be accounted for by a minimal 3-state model", the choices made for the model (which rates are force dependent, which are not, which ones are dependent on inter-head strain) could be explained in more detail.
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For example, ChR2 mutants with greatly extended lifetimes of the open channel state have been generated.
Popular with extended families.
In the past few years cellular signaling pathways that interact with these hormonal pathways have yielded many mutants that extend lifespan in non-mammals.
Although deletion of swi6 has been previously identified as having a synthetic negative effect with cohesin mutants, we have extended these finding to include subunits of the CLRC and RDRC complexes, which function in different aspects of heterochromatin function and have even stronger synthetic effects on growth.
To test whether the metabolic instability of the Ura3 mutants could be extended to a fusion protein, we tagged them with GFP.
Similarly to the other mutants, mrc1 deletion extended S-phase.
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