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We have investigated how mutants with enhanced stomatal closure behave in a competitive situation with wild type plants for water.
CYP102A2 was subjected to error-prone PCR to generate mutants with enhanced activity with fatty acids and other aromatic substrates.
As a result, four mutants with enhanced catalytic activity toward levulinic acid were obtained, and the most improved mutant, Met13Trp, exhibited a 7.0-fold increase in catalytic efficiency.
In the same manner, about 200,000 mutants of PHA synthase gene from Aeromonas punctata were screened and only five mutants with enhanced fluorescence were isolated (Amara et al. 2002).
Our results show that BP-Dock is a useful tool for rapidly screening the relative binding affinity pattern of in silico-designed mutants compared with wild-type, supporting its use to design novel mutants with enhanced binding properties.
Col-0 mutants used in this study include those deficient in lignin, cellulose, components of hemicellulose such as xylose and fucose, the pectic components arabinose and rhamnose, as well as mutants with enhanced arabinose and total pectin content.
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A nine-fold mutant with enhanced catalytic activity in the hydrolysis of cephalosporin C to 7-aminocephalosporanic acid, but with low stability, was used as a starting point.
A bacterial mutant with enhanced PHB production can be distinguished from randomly mixed samples such as random mutagenesis by error-prone PCR in a high-throughput manner using LipidGreen1.
Dania Ramirez as Callisto The leader of The Omegas, Callisto is a mutant with enhanced superhumanly acute senses, who senses mutants and their powers, and possesses superhuman speed.
We also identified a TILLING mutant with enhanced fruit shelf life.
Since the low thermodynamic stability of CspB (ΔGD = 12.4 kJ/mol) might be responsible for unfolding and integration of CspB into fibrils, fusions with a CspB mutant with enhanced thermodynamic stability (ΔGD = 26.9 kJ/mol) were studied.
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