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Mutants with elevated Aza activity were identified, DNA sequenced, and the proteins purified.
E539K and K312E/E539K were the two mutants with elevated optimal temperature (Fig. 2c) and enhanced thermostability (Fig. 2d).
Mutants with depressed GLR-1 synaptic levels rarely reverse direction, whereas mutants with elevated synaptic levels reverse direction with increased frequency [47] [49], [51], [52].
Unexpectedly, when a vector, which utilizes this phenomenon, was used to produce mutants with elevated activity of NF-κB, we found mutants, which owed their phenotype to the effect of the insert on a gene located tens or even hundreds of kilobases away from the insertion site.
Similarly, C. albicans cell wall mutants with elevated chitin contents are less susceptible to caspofungin (Plaine et al., 2008).
The xprG gene was first identified in a genetic screen for mutants with elevated proteases secretion (Katz et al. 1996).
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The novel mutant allele hsi2-4 was isolated in a genetic screen to identify Arabidopsis mutants with constitutively elevated expression of a glutathione S-transferase F8::luciferase (GSTF8 LUC) reporter gene in Arabidopsis.
In all of the independent mutant lines with elevated seed stearic acid, SACPD-C mutations also resulted in nodules with very atypical nodule structure.
Strikingly, loss of intact AJs in crb mutants also correlated with elevated apical actin and increased cell protrusiveness (Fig. 6F F″, arrows), as we observed in embryos expressing Rab11DN (Fig. 4K).
Increased LUC transcript levels in those mutants are correlated with elevated luciferase signals.
Unlike wild-type or (66delD Cx31, the expression of the EKV mutants are associated with elevated levels of in vitro cell death (21– 23) through a mechanism which remains to be elucidated.
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mutants with wild
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mutants with corresponding
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