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Competition assays were used as a more stringent measure of fitness differences in mutants with different codon pair biases.
CpG and UpA dinucleotide frequencies primarily influenced virus replication ability while no fitness differences were observed between mutants with different CP usage where dinucleotide frequencies were kept constant.
Children recognised both sets of mutants with different age groups having different recognition levels.
A number of TEVp mutants with different rate of cleavage, stability and specificity have been reported.
As the most obvious result of these experiments two groups of mutants with different growth and accumulation behavior were revealed.
To adapt the protease to diverse applications, several TEVp mutants with different stability and enzymatic properties have been reported.
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Studies on the four STT3 genes of L.major showed that three of them complement the yeast Δ stt3 mutant with different efficiencies, that they can function without association with other yeast OTase subunits (Nasab et al, 2008; Hese et al, 2009) and that they have overlapping but distinct peptide substrate specificities (Nasab et al, 2008; Hese et al, 2009).
Four independent homozygous mutants (KO1-4) with different mutation types were obtained, and all mutations results in premature stop codons (Fig. 3b).
The genotype of such mosaic individuals can contain several mutant alleles with different frequencies and each mutation can be homo- or heterozygous at the cellular level.
Student's t-test (paired, two-tailed) was used to identify mutants with significantly different sensitivity relative to wild type.
In addition to mutants with drastically different morphologies, quantitative studies will benefit from mutants with subtle changes in cellular dimensions, providing the opportunity to systematically tune length and width in any study of a given phenotype.
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