The simulation result is, however, in agreement with the recent NMR detection of folded monomers of HIV-1 PR mutants with a destabilized interface.
Isothermal equilibrium denaturation measurements showed that the Q53T and Q53D mutants were destabilized, whereas the Q53N mutant was stabilized relative to FKBP∗ with little change in the equilibrium m values.
All MftR mutants were destabilized compared to WT MftR.
Overall for the restorative mutants, a destabilized helix 3 was observed in the V266H single mutant, but no rotation in the helix was observed in the F128A/V266H, Y195A/V266H, and Y195F/V266H double mutants.
The P. berghei orthologs of the identified P. falciparum genes were compared with a global gametocyte transcriptome analysis of P. berghei DOZI and CITH gene deletion mutants; destabilized mRNAs of these mutants are candidates for translational repression in non-activated gametocytes [ 19, 20].
Together with the ubiquitin ligase Ubr1, Rad6 ubiquitylates proteins with destabilizing N-termini [ 4].
Previous work indicates at least some PMP22 disease mutants are considerably destabilized; even wild-type (WT) PMP22 is only marginally stable, being transported to cell plasma membranes with an efficiency of only ∼20%.
Teschke's group has also carried out elaborate experiments which establish that these ts mutants are indeed destabilized (S.M. Doyle et al. JBC 2004).
Mutants known to destabilize the iNOS dimer (E411A, D454A, and W188F) were also investigated; in these cases co-expression with the wild-type subunit did not lead to the formation of stable mixed dimers.
The evidence indicating a role for SPI-2-associated F-actin in stability of the vacuolar membrane came from experiments in which membranes enclosing wild-type but not ssaV mutant bacteria were destabilized when infected cells were treated with the actin-depolymerizing drugs cytochalasin D or latrunculin B (Méresse et al., 2001).
