However, the CA loop region cannot solely explain the resistance of SIVMAC to the TRIM5αrh-mediated late restriction, because an HIV-1 cyclophilin-binding loop mutant with corresponding SIVMAC sequence was still sensitive to the late restriction [36].
(B ) Side-by-side comparison of human and Drosophila CL mutants, along with corresponding WT constructs.
Secondly, lamellocyte differentiation in response to Salmonella infection is blocked in decapentaplegic mutants with a corresponding increase in plasmatocyte number, suggesting that lamellocytes arise from plasmatocytes or a common precursor [23].
Our initial attempt to complement these mutants with their corresponding wild type gene on a multicopy plasmid vector was unsuccessful, presumably because of the toxicity of uncontrolled overexpression of these gene products.
At the third level we directly compared aphid-induced transcriptional changes in each of the mutants with the corresponding changes in wt plants.
To probe the binding sites of Nav1.2 and Nfasc on AnkG, we constructed AnkG_repeat mutants with the corresponding hydrophobic residues in binding site 1 (Phe131 and Phe164 in R4 and R5, termed 'FF'), site 2 (Ile267 and Leu300 in R8 and R9; 'IL'), and site 3 (Leu366, Phe399, and Leu432 in R11, R12, and R13; 'LFL') substituted with Gln, and examined their binding to the two targets.
We mixed mutant plasmid with corresponding wild type plasmid at various ratios and amplified the mixed plasmid as a template of PCR using paired primer sets for mutational analyses.
Data compiled in histograms summarize the initial and net SNP-induced conductance data for mutant channels, with corresponding wild type and control responses assessed from within the same batches of oocytes (Figs. 5, 6).
Each mutant with the corresponding GFF2 data was visualized against the rice genome as a separate track.
An amp1 mutant was found to be deficient in stimulating miRNA activity, and intriguingly, miRNA target protein levels were elevated in this mutant with no corresponding changes in target mRNA levels.
