Johnson K, Lory S. Characterization of Pseudomonas aeruginosa mutants with altered piliation.
Utilizing TraI-YFP cloned into the i31 sites, we created YFP point mutants with altered stability, G67A and I161A.
Rice mutants with altered response to diseases have been found (Campbell and Ronald 2005; Wang et al. 2004).
Mutants with altered response to pathogens, either gain or loss of resistance, are particularly useful for dissecting defense mechanisms.
Randomized sequences are then cloned into a suitable expression vector, and the resulting mutant libraries can be screened to identify mutants with altered or improved properties.
Other rice mutants with altered root gravitropic responses have been isolated: RM109 (Hao and Ichii 1999) and aem1 (Debi et al. 2003, 2005).
IGFBP-2 mutants with altered IGF binding-, RGD-, and heparin-binding sites were generated and their actions examined.
ATR1/MYB34 was identified as a gain-of-function mutant, atr1D, in a screen for mutants with altered tryptophan regulation [8], [9].
Indeed, recent studies have observed decreased virulence and enhanced innate immune responses for F. tularensis subsp. novicida mutants with altered lipid A moieties [57].
One-exon transdominant Tat mutants with altered or deleted basic domains have previously been shown to localize predominantly to the cytoplasm of cells [3], [9], [10].
The response ratio of the deAmTracs correlated with transport activity in mutants with altered capacity.
