Genome scale metabolic modeling is shown to predict overexpression and deletion mutants which enhance recombinant protein production with high accuracy.
In contrast, cells expressing human ZnT3 carrying the Y357F mutant, which enhances ZnT3 dimerization, showed a modest but significant increase in cell viability compared with human wild type ZnT3 (Fig. 5E).
We have elucidated genes not previously associated with EGFR-mutant NSCLC, two of which enhanced the clonogenicity of these cells, distinguishing these mediators from others previously shown to maintain cell survival.
The results suggested that an induction mechanism was involved in the regulation of β-Xylosidase biosynthesis which enhanced specific yield of enzyme (Yp/x) up to 59-fold in the mutant cells (Rajoka and Khan 2005).
It seems unlikely that the sacy-1 missense alleles have an enhanced sensitivity to RNAi because even rrf-3 (pk1426 ) mutants, which exhibit enhanced RNAi responses (Sijen et al. 2001), only exhibit a moderate increase in embryonic lethality after sacy-1 (RNAi).
Finally, S1 pyramidal neurons in glycine transporter heterozygote (GlyT1+/−) mutants, which display enhanced NMDAR function, had increased dendritic spine density.
This inhibitory effect of Thr-469 was further evidenced in T468A/T469A (lane 9) and T474A/T469A (lane 11) double mutants, which exhibited enhanced substrate phosphorylation in comparison with NIK1, in spite of harboring mutations in the relevant Thr-474 or Thr-468 residues for autophosphorylation (Figures 7B and 7C).
We evaluated the involvement of putative chitin receptor gene HvCEBiP in barley basal resistance using the mossd1 mutant of Magnaporthe oryzae, which enhances host basal defense responses.
Here, we use compound mutant and lineage-restricted mice to demonstrate that Nf1+/- macrophages produce excessive ROS, which enhance Nf1+/- smooth muscle cell proliferation in vitro and in vivo.
Interestingly, the E159A HCCS mutant, which exhibited enhanced function, also copurified with substantially less heme compared to WT HCCS.
