Here we report highly potent dual small molecule inhibitors of both ALK and EGFR, particularly the T790M mutant which confers resistance to first generation EGFR inhibitors.
With the exception of the M130I Vmutant8) mutant, which confers a 2-fold increase in resistance to bafilomycin, V-ATPase mutants did not show cross-resistance to either bafilomycin or concanamycin.
It is possible to presume that the higher apoptotic rate in control plant cells after nocodazole treatments may be also a consequence of absence mutant microtubule protein(s) or mutant microtubule associated protein(s) which confer(s) resistance to IPC in N. sylvestris mutant lines.
An Arabidopsis CAX1 mutant (CAXcd), which confers enhanced Cd transport in yeast, was ectopically expressed in petunia to evaluate whether the CAXcd expression would enhance Cd tolerance and accumulation in planta.
We also employed the LQT mutant KCNE1-L51H, whiconfersers a misfolding-dependent trafficking defect [30], [33].
By contrast, the 'IY' mutant of CRY1, which confers severe structural disruption in the C-terminal helix, failed to restore any level of circadian rhythm, although it has the ability to repress CLOCK-BMAL1 as seen by the constitutively low luciferase signal.
In particular, 34c was potent against a variety of frequently observed crizotinib-resistant mutants, particularly the L1196M mutant (IC50 = 3.1 nM) identified as the "gatekeeper" mutation and the G1202R mutant (IC50 = 8.7 nM) which conferred resistance to all clinical stage ALK inhibitors.
The allele n137 is a gain-of-function mutation in lin-12, which confers ectopic vulval induction in mutant hermaphrodites.
ATP deficiency, specifically at synapses, has also been suggested to underlie the phenotype of a second mutant allele of sesB, sesB org, which confers temperature-sensitive paralysis (Rikhy et al., 2003).
The vector pCCBX-YR contains sdi1 R gene (1008 bp) encoding mutant allele of the succinate dehydrogenase, which confers resistance to the fungicide carboxin, under the control of Z. tritici sdi1 promoter (1027 bp) and the sdi1 terminator (197 bp) sequences (Fig. 3).
