This Zn content is similar to the levels seen in the hma4 mutant, which appears similar to wt in most growth parameters.
Summarized normalized curves are depicted in figure 5A C, while pEC50 values are reported in table 2. As shown in figure 5B, the DRY/AAY is the only mutant, which appears practically abrogated in signaling capacity.
One surprise was the mild phenotypic changes observed for the L150K mutant, which appears to have only a small effect on the MyoD-Id2 interaction despite near conservation of leucine at this position throughout the bHLH family.
CIFM analysis revealed that the S217A mutant codistributed with melanosomes; however, this was less obvious for the G440A mutant, which appeared more uniformly distributed throughout the cytoplasm, possibly due to its increased affinity for actin.
Unlike the Li2 mutant which appears healthy and morphologically identical to the homozygous recessive wild-type plants with the exception of shorter seed fibers, the Li 1 mutant exhibits pleiotropy in the form of severely stunted and deformed plants in both the homozygous dominant and heterozygous state [ 23].
In particular, it will be interesting to further characterize class 2 AR mutants (Table 1), which appear to play stronger roles in responses to the inhibitory effects of ABA on root elongation than on seed germination.
These compound mutants have accentuated degenerative phenotypes, which appear at earlier generation times, and suffer additional pathologies, such as bone loss and kyphosis (Chang et al, 2004; Du et al, 2004).
Li et al (2007) also described apparent disruptions in cell adhesion between presynaptic neurons and postsynaptic muscle in larval neurexin mutant NMJs, which appeared as widened synaptic clefts visible by electron microscopy [37].
The most drastic effect was observed with HN mutant 262+461, which appeared to be released without any HN proteins.
Triclosan, an inhibitor of FabI (an enoyl-acyl carrier reductase) and a component of fatty acid biosynthesis [ 57– 59], shows differential inhibition of the mutant clones, which appears to be opposite of that found by pro-oxidants.
