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Consistent with photoreceptor alteration, PNA labelling of cone outer segments was reduced in mutants, which also showed absence of rhodopsin at P120 (Fig. 3A).
Kif3a mutants, which also lack all cilia due to the absence of the Kinesin-II anterograde IFT motor, arrest at e9.0, somewhat earlier than Ift88 and Ift172 null mutants.
Figure 3 shows several examples of EF-Tu interface mutants which also show kpep > 0.25/s and thus are also rate limited by product release.
However, the phenotypes of altered axonal pathfinding and ectopic branching were different from the motoneuron phenotypes observed in zebrafish you-too (yot) mutants, which also lack adaxial cells.
The production of lactate, ethanol and acetate was different in ldhA, adhE, and pta deletion mutants, which also proved this point.
This phenotype of reduced bristle size is reminiscent of myc hypomorphic mutants, which also display smaller and thinner bristles (Johnston et al. 1999).
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A similar down-regulation of these genes is seen in the ms1 mutant, which also presents defective late pollen wall and coat development [38].
However, the full-length β-gal is synthesized significantly only by the double mutant, which also encoded correspondingly less of the truncated protein (Figure 4C).
Specifically, the only strain derived from RN6390 that produced a biofilm in our microtiter plate assay was an agr mutant, which also exhibited decreased production of extracellular proteases (Fig. 6).
The ems63089 mutant shares some similarity with the mac1 mutant, which also fails to differentiate tapetal and middle layers.
A similar effect was found with the C86F mutant, which also resulted in a UVS pigment (365 nm, Figure 2B).
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