Sentence examples for mutants when shifted from inspiring English sources

For determining the phenotypes of the TS mutants when shifted to the restrictive temperature from the L1 larval stage, we performed hypochlorite treatments and allowed the embryos to hatch in M9 buffer at 15°C.

We find that 62% of the mutants display additional phenotypes when shifted to the restrictive temperature as L1 larvae, in addition to causing embryonic lethality after L4 upshifts.

Likewise, orc5-1 H3 (K9, 18, 18, 23, 27R) mutants were completely inviable when shifted at 30°C (see Additional file 6).

The phenotype of Su(dx) null mutations is also temperature sensitive and, unlike WT, the mutants upregulate N signaling when shifted up to 29°C (Fostier et al., 1998; Cornell et al., 1999; Mazaleyrat et al., 2003).

The tba-1 or594 tba-1 or594nt sdowed highly penetrant defects when shifted to the restrictive temperature for long (5–8 hours) or short (∼1 minute) upshifts,tshile the tbb-2(or600 sd,ts) mutant showed penetrant defects only after long upshighly(Fig. 1C and Table 3).

Similarly, tac-1 lf) tac-1 lfdisplayed reduced PLmutantsregrowth when shiftedisplayed to 25°C in the L1 stage, 20 hreducede axotomy; tac-1(lf) animals raised at 15°C disPLMyed normaxonegregrowthot showhen

The evidence for this interpretation includes the observations that ire1Δ and hac1Δ mutants fail to sustain expression of INO1 when shifted to medium lacking inositol and that the opi1Δ mutation supresseses their Ino− phenotypes (Cox et al. 1997).

One of the spd-2 alleles was fast-acting, or293 ts, while or493 ts and or454 ts mutant embryos showed weak and only partially penetrant defects when shifted to the restrictive temperature for 1 minute (Fig. 6C and Table 3).

Instead of a functional inflorescence with leaves and flowers, eif3h mutant plants sometimes formed a pin-formed shoot, especially when shifted from short-day to long-day growth conditions (20%, n = 90) (Fig. 3A).

Consistent with the strong neuronal expression, Drosophila shibire temperature sensitive mutants show a paralysis phenotype with impaired synaptic vesicle (SV) recycling when shifted to the non-permissive temperature (Grigliatti et al., 1973).

When shifted to non-permissive temperature during exponential growth, these mutant cells specifically lose actin cables within a few minutes [18].