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Transcript and metabolite profiles of the wild-type (WT) strain compared to the above-mentioned mutants indicated that the photorespiratory mutants, when grown under HC conditions, display gene expression and metabolite patterns that are characteristic for WT cells when shifted from HC to LC conditions [15], [16].
Petiole elongation is strongly promoted in gi mutants when grown in short days, as seen for gi-1 adult plants.
A leading phase was previously observed with phyB single mutants when grown on MS medium (Hall et al, 2002; Salomé et al, 2002).
At a molecular level, global H4 acetylation is dramatically reduced in esa1 mutants when grown at restrictive temperatures (Clarke et al. 1999).
M. bovis BCG WT and the mutants were grown in Middlebrook 7H9 broth (Difco) supplemented with 0.05% Tween-80, Middlebrook Oleic acid albumin dextrose catalase (OADC supplement, Becton Dickinson) and antibiotics (100 µg/ml streptomycin for WT and additionally 50 µg/ml kanamycin for the mutants) when grown in liquid culture.
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Moreover, no phenotypical differences in the stress response were observed between the wild type and the single and double knockout mutants when growing in the presence of high salt or osmotic stress, or after incubation at 37°C.
However, spoT was up-regulated in our C. jejuni Δppk2 mutant when grown in rich media (Figure 6).
We also investigated filamentous growth of the age3 mutant when grown on Spider agar and when embedded in YPS agar (Fig. 5).
Competitive growth experiments were undertaken to assess the viability of the SH1000 σS mutant when grown in coculture experiments with its parental strain SH1000.
(C ) In PA1, the double mutant besA E2/ clcA E2 has similar fitness to the besA E2 single mutant when grown on DCM.
The rate of xylose uptake was faster in the wild-type strain than in the creAd30 mutant when grown on xylose as a sole carbon source.
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