Additionally, Salmonella cra mutants are avirulent when administered orally, indicating that Cra may have key roles in enteric pathogens in anaerobic conditions [ 74].
We have also shown that the rapamycin analogue temsirolimus (cell cycle inhibitor-779) reduces aggregate number and behavioural abnormalities in a transgenic mouse expressing a mutant huntingtin fragment when administered ∼6 weeks before the onset of signs (Ravikumar et al., 2004).
For instance, a mutant derivative of LT, named LT R192G), when administered intra-nasally with viral epitopes/antigens was shown to induce mixed Th1 and Th2 cellular immunity, and CD8+ CTL responses [24] [27].
Colonization analysis also revealed the incapability of the ΔsalKR mutant to colonize any susceptible tissue of piglets when administered alone.
Additionally, a Y. pseudotuberculosis pil – mutant was delayed in the killing of mice when administered orally, however it was as virulent as the parental strain when administered intravenously.
We have now shown that BB-10010, a stable mutant of MIP-1alpha, (a) is more effective when administered as a continuous infusion than when bolus injected and (b), when administered via a 7-day infusion during and after cyclophosphamide treatment, results in an earlier recovery of leucocyte numbers.
Colonization experiments showed that the ΔsalKR mutant could not colonize any susceptible tissue of piglets when administered alone.
When administered at high doses, S. Typhimurium rpoS mutants can protect against homologous, but not heterologous challenge [26], [27].
When administered ID to BALB/c mice the SCHU S4 deletion mutants provided better protection than LVS against a subsequent low dose aerosol challenge with the wild-type strain [21].
The protein fadD26 (Rv2930) included in our selection, is a necessary part of the operon for biosynthesis of DIM [ 153], and its mutant is attenuated in mice and induces better protection than BCG when administered sc.
