Sentence examples for mutants were without from inspiring English sources

Our results illustrate that spine density was decreased following ectopic expression of both wild-type PTEN and the protein phosphatase-competent G129E mutant; in contrast, catalytically dead (C124S) and protein phosphatase-deficient but lipid phosphatase-active (Y138L) mutants were without effect.

In contrast to MT1, overexpression of the E240A mutant was without effect, indicating the requirement for MT1 catalytic activity in the enhanced invasion.

Both simple mutants were obtained without any major difficulty and were confirmed by Western Blot analysis (Figure 2A, lanes 1 3).

(E ) Ba/F3 cells transfected with WT p85α or mutants were cultured without interleukin-3 for 4 weeks and harvested for viability assays.

Both mutants were designed without the N-terminal ELR motif in order to prevent receptor signalling while keeping the receptor docking domain untouched.

(D ) Ba/F3 cells transfected with WT p85α or mutants were cultured without interleukin-3 for 4 weeks and harvested for viability assays.

As expected, the observed NF-κB signalling activity was dependent on the presence of the HOIL-1L interaction domain, the ubiquitin-associated domain (UBA domain) in HOIP, as well as on the co-expression with HOIL-1L, since neither wild-type nor HOIP mutants were active without it (Supplementary Figure S6B).

(C) 293T cells harboring the expression plasmids of PML-RARA mutants were treated with or without 2 µmol/L AS2O3 for 4 h.

glo-4, ppm-1 double mutants were constructed by recombination without using visible markers.

In brief, Ba/F3 cells transfected with WT PIK3R1 or mutants were cultured in medium without IL-3 for 4 weeks.

Single cross-over mutants were grown in TSB without antibiotic at 30°C overnight, diluted 1 100,000 in sterile water and 100 μl was spread on TSA plate containing 50 μg/ml anhydrotetracycline and incubated at 37°C overnight.