Sentence examples for mutants were verified from inspiring English sources

Δtri11 and Δtri4 mutants were verified with the same method (Figs. 2b and c).

Potential mutants were verified by PCR and Southern blot hybridization.

The PCR-amplified regions of all mutants were verified by DNA sequencing.

The obtained mutants were verified by PCR, using the appropriate primers (Table S1), and by nucleotide sequencing.

Chromosomal loci of the generated mutants were verified by PCR using a primer specific to the insert and a primer that annealed to sequence that flanked the disrupted loci (Table S2).

All mutants were verified by DNA sequencing.

The btcA−bteA region of the mutants was verified by DNA sequence analysis.

Each of the mutants was verified by PCR and sequence analysis.

The integrity of the RFM mutants was verified by DNA sequencing.

The entire open reading frame of all shs1-ps mutants was verified by sequencing.

The integrity of the MENT mutants was verified using: (1) far-UV circular dichroism spectroscopy (data not shown); (2) thermal stability; and (3) inhibitory activity (the association rate constant, kass, and the stoichiometry of inhibition, SI) against human cathepsin V (Table 2).