Sentence examples for mutants were transformed from inspiring English sources

The binary vector was transformed into Agrobacterium tumefaciens strain EHA105 (Hood 1993) by electroporation, and Srs5 mutants were transformed as reported previously (Ashikari et al. 2005).

pET28a vectors containing ORF of xtGadd45a and mutants were transformed into BL21DE3 E.coli.

Plasmid DNA containing mCerulean mutants were transformed into XL10 bacteria and colony fluorescence was examined by fluorescence microscopy.

Centromeric plasmids pSW72-733 and their derivatives) containing these mutants were transformed into a strain heterozygous for deletion of CDC24 (RLY2775).

The ETR1(C6S, C6S), ETR1(C4S) and ETR1(C6S) mutants were transformed into an etr1-6 etripleein4-4 Arabidopsis triple mutant background, their ability to rescue the mutant phenotype indicating that they are functional receptors.

The wild-type allele of each mutant gene was isolated and identified by complementation cloning: mutants were transformed with a genomic library and colonies able to grow on glycerol were selected.

Due to some genes with multiple insertions (Table 1), this pool was reduced to 12 insertions in unique loci and each of these mutants was transformed with plasmid pAJ22, which constitutively expresses the lacZ gene.

Each construct (WT and mutants) was transformed into E. coli BL21 and expressed using an auto-induction method at 25°C for 16 h [ 41].

PTP1B pRSET-C PTP1B pRSET-Cruct and mutants was transformed into SoluBL21 Escherichia coli (Amsbio, Lake Forest, CA, USA), and heterologous plasmid expression was induconstructlture in ando-induction mutants(Formedium, Norfolk, UK), at 37 °C for 24 h.

Both Col-0 plands and the atl9-1 mutant were transformed using the floral dip method [72], [73].

E. coli strains BW25113 (wild type) and JW2861-1 (Δ dsbC mutant) were transformed with pBAD33 (as control) or pBAD-AppA.