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These mutants were transferred from the solid to liquid medium and kept under phototrophic growth conditions (photon flux density 120 μmol photons m−2 s−1).
After overnight growth, the mutants were transferred on Q-Tray bioassay plates containing LB solid medium (Additional file 1: Fig. S1b, gridding) and activity was detected after Congo Red staining.
After overnight growth at 30 °C, the mutants were transferred on 22 × 22 cm Q-Tray bioassay plates containing LB-agar containing ampicillin (100 μg/mL) supplemented with 0.2% w/v carboxymethylcellulose (CMC) as substrate and 1 mM Isopropil-β-d-1-tiogalattopiranoside (IPTG) as inducer and the incubation was carried out overnight at 28 °C.
The germline-deficient mutants were transferred at the same time as the N2 wild-type to maintain consistency.
To test whether the induction of PCD could be attributed to the reduced myo-inositol or galactinol accumulation, atips1 mutants were transferred to LD, and either sprayed with a 100 mg/mL myo-inositol solution or brushed with a 10 mM galactinol solution.
The remaining mutants were transferred to soil for seed production.
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Full length mouse nucleolin cDNA and NLS nucleolin mutant were transferred from pcDNA3.1 into pWPTS-adapter in SalI and SpeI sites.
Bacterial mutant were transferred on the ice to remove thermal radiation, and protect enzyme from inactivation.
The Mdr2-KO mice develop liver tumors in about 100% of animals by the age of 16 months on the FVB/N genetic background; initially, this model was generated in the 129/OlaHsd strain, but due to a low fertility of the resulting mutants, the Mdr2-KO mutation was transferred into the FVB/N strain [11], [18].
We showed previously that this abnormal pattern of starch turnover was rapidly regained in lsf1 mutants that were transferred to normal light/dark cycles after starch was reduced to very low levels by prolonged darkness (Comparot-Moss et al., 2010).
A significant reduction in the context-specific modulation of firing rate was observed in the CA3 pyramidal cells when the mutant mice were transferred from one context to another.
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mutants were confirmed
mutants were recovered
mutants were obtained
mutants were discovered
mutants were generated
mutants were set
mutants were monitored
mutants were named
mutants were indicated
mutants were affected
mutants were expressed
mutants were designed
mutants were solved
mutants were screened
mutants were compared
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