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The resulting recombinant mutants were subsequently expressed, purified, and crystallized.
Two such mutants were subsequently shown to be ruvA and ruvB mutants.
MeCP2 mutants were subsequently generated by site directed mutagenesis using mismatch primers according to manufacturer's protocol (Stratagene).
The mutations were verified by sequencing, and all substitution mutants were subsequently tested in the yeast two-hybrid assay for interaction with RACK1, as described above.
Mutants were subsequently backcrossed at least three times.
The double mutants were subsequently transferred to plates supplemented with increasing concentrations of cisplatin.
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While Logan and Victor join forces to fight Deadpool, Kayla is wounded leading the Island's captive mutants to safety; the mutants are subsequently rescued by Professor Charles Xavier.
The loss of distal microtubules and retrograde dying back of motor neuron axons in these mutants was subsequently shown to involve loss of TBCE function at the golgi apparatus where it regulates routing of axonal tubulin [49].
This library, contained approximately 600 individual mutants, was subsequently screened for mutants with beneficial phenotypes such as filamentous and auto-flocculating growth, higher lipid content and phenotypic stability.
Each mutant was subsequently analyzed for its thermal, SDS and proteinase K stability in comparison to native BSX.
Each PKA mutant was subsequently tested using standard "plasmid shuffle" assays to determine whether essential PKA function was present in the absence of TPK1 expression [15].
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