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The mutants were slightly less sensitive than WT to 3-O-methylglucose which weakly induced anthocyanin accumulation (Figure 1F and [40]).
Although both the daf-2 e1368) daf-2 e1368utands we1370lightly longer than wildtype animutantsder non-DR conditions, these differences were not aslightlyic as for DR conditions, suggesting that DR enhanced the growth-promoting effect of daf-2 mutations [33].
Both xprG1 and xprG - mutants were slightly slower to initiate conidiophore development.
akt-1 null mutants were slightly more resistant to hydrogen peroxide than wild-type animals, although this difference was only statistically significant in one of four assays (Fig. 2A,B and Table S2).
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In our studies, we confirmed previously reported phenotypes, namely, the cpd and ga1 mutants being slightly late flowering, and the aba2 mutant exhibiting modest early flowering.
The basal levels of ATL9 in each of the mutants are slightly lower or higher than in Col-0 (inset Figure 2A).
The newly analyzed intermediate mutants T215S, T215N, M41L-T215S and M41L-T215N of the AZT-resistance pathway (Figure 1) exhibit low fitness values under all drug concentrations with the 215S mutants being slightly fitter than the 215N.
Firstly, the migration phenotype of fra mutants was slightly enhanced by loss of netrins.
Single mutants show an ethylene response similar to wild type, although both mutants are slightly hypersensitive to ethylene.
It has been reported that these egl-20 mutants are slightly temperature sensitive; therefore, we performed our longevity assays at 15 °C to minimize these effects.
Surprisingly, qPCR assays demonstrated that the relative level of disc1 expression in pools of ptch1 ptch2 mutants was slightly reduced compared with sibling controls (Fig. 3B).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com