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Approximately 100 mutants were segregated and confirmed to be homozygous.
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The WT and mutant were segregated from the same Tos17 insertion line NG6441 following genotyping using the primers F1 and R1 for WT allele, and F1 and T5 for the mutant allele (Additional file 1 Table S1 Table
Detailed phenotypic analyses of bak1-SUP1 and a single mutant in which the bak1 mutation was segregated out (miR172-D) revealed that the overexpression of miR172 promotes leaf length elongation in adult plants and increases the root and hypocotyl growth during the seedling stage compared with that of wild type plants.
Although this finding supports the conclusion that the migration defects can be explicitly attributed to the mir-34 and mir-83 loss of function, a caveat remained that the migration defect was due to background mutations, outside the mir-34 or mir-83 loci, that had been present in the single mutants and were segregated away during construction of the transgene-containing strains.
Biallelic T2 mutants for ETC2 were segregated from only two T1 lines among the five lines examined (Table 1), demonstrating that the frequency of germline transmission of the ETC2 mutations into T2 plants was much lower than that of the TRY and CPC mutations.
Hotels were segregated.
"Carousels were segregated.
Our schools were segregated.
Planktonic microorganisms were segregated into latitudinal belts.
In contrast, clusters 1 and 2 are enriched for cells with EGFR mutations; within each of these clusters, cells with wt and mutant EGFR are segregated.
By analyzing the recruitment of the plus-end protein astrin, we show that a pure-lateral attachment (as in HEC1Ndc80-Δloop-mutant-expressing cells) could be segregated from a mixed-lateral attachment (as in MCAK-depleted cells).
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