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Selected mutants were scored for LBI every other day under the Fe toxic treatment (Figure 3).
At the 6th day, the heterozygous (GFP+) and homozygous (GFP−) mutants were scored.
All of the mutants were scored in at least three independent experiments.
Plates were allowed to dry before being inverted and incubated at 37°C for up to 72 hrs before mutants were scored for defects in forming L-form colonies.
Potential ry mutants were scored by crossing to v ;ry.
Two independent lines of tert/nbs1 double mutants were scored, K12 and L2.
Similar(45)
The cytokinesis defect of spc105 mutants was scored following zymolyase treatment [57], and by tracking septin ring duplication (Cdc3p-GFP [9]).
Transgenic Roller progeny were isolated and propagated; rescue of bas-1 mutants was scored by staining with serotonin antiserum as described previously[ 16, 67].
In the first method, the frequency of purR mutants was scored unselectively, using a tester strain that carried a stem-loop deletion (mDEL1).
With SGA analysis, we quantify both negative and positive genetic interactions, where double mutants are scored as growing worse or better than expected, respectively (Baryshnikova et al. 2010).
Some of the genes tested by genetic null mutation also may be reported as false negatives in our screen due to maternal rescue in cases where homozygous sterile mutants are scored from heterozygous mothers (see below for discussion of drsh-1 /Drosha).
More suggestions(15)
variants were scored
ones were scored
mutants were confirmed
mutants were obtained
mutants were recovered
mutants were discovered
mutants were generated
mutants were set
mutants were monitored
mutants were indicated
mutants were affected
mutants were named
mutants were expressed
mutants were designed
mutants were solved
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