Overall, our rps19 model, as well as rpl11 mutants is responsive to L-leucine treatment.
In order to determine whether spt mutants are responsive to reduced levels of GA, we exposed seedlings to the GA biosynthesis inhibitor paclobutrozol (PAC) and examined the effect on meristem size.
Consistent with this property, Dosil et al. found that the Ste2-F204S mutant cells were responsive to high α-factor concentration in cell division arrest (halo) assay and in α-factor induced transcription assays (Dosil et al., 2000).
To determine whether the mutant constructs were responsive to GSMs and iGSMs, GSM-1, a piperidine acetic acid as a potent acidic GSM, Compound 2 (Cmpd2), a piperazinyl pyrimidine as a potent nonacidic GSM, and fenofibrate, one of the more potent iGSMs identified, were utilized.
Sixteen of the mutant forms that were responsive had AT1001 EC50 values of less than or equal to 10 µM (Table 1 and Fig. 2).
In this comparison, 90% of the mutant forms that were responsive in male Fabry patient lymphoblasts were also responsive in HEK-293 cells, whereas 92% of those that were nonresponsive in male Fabry patient lymphoblasts were also not responsive in HEK-293 cells.
PDGFRA mutants were not responsive to their ligand, PDGFAA, and displayed a high constitutive kinase activity.
Third, the ssn8 mutants were still responsive to light and their mating filaments were inhibited by light (data not shown).
None of the mutants or wild-type were responsive to toluene either.
