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We further examined whether defects in the flying ability of tw mutants were rescued by overexpression of the tw gene.
Moreover, the barrier defects of acs-20 and acs-20 acs-22 acs-20 acs-22 acs-20 acs-22-20, acs-22, or humutantsp4 transgenes.
By picking first instar larvae and seeding them at fixed density on standard flyfood, we found that 83% of control w1118 larvae survived to adulthood, and 69% of Tsc129 mutants were rescued to adulthood with the Tsc1WT transgene (Figure 2B, "WT").
We then chose transgenes expressing Tsc1S533A or Tsc1S533D at levels similar to Tsc1WT (Figure 2A, lanes 2, 4 and 5), introduced them into the Tsc129 background, and found that they were also able to rescue the mutant flies as efficiently as the Tsc1WT construct: 61% and 63% of Tsc129 mutants were rescued to adulthood with the Tsc1S533A and Tsc1S533D transgenes respectively (Figure 2B).
We found that the temperature-sensitive growth, the cell lysis defect and the morphological defect of the mutants were rescued by 1 M sorbitol, an osmotic stabilizer, known to rescue cell wall defects, suggesting that the primary defect might be a defective cell wall structure (Fig. 4A, B).
The odor receptor mutants were rescued using transgenic expression of odor receptors, validating assignment of phenotypes to the alleles.
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Interestingly, the mislocalization of Myospheroid in Rac1 mutants is rescued by hyperthermia, involving the heat shock protein Hsp83.
We also showed that the barrier defects of acs-20 and acs-20 acs-22 acs-20 acs-22 acs-20 acs-2220, acs-22, and humutantsp4 traregenes.
Expectedly, homozygous porinA2 (A2) phenotype was strongly suppressed by Drp1 overexpression or Marf knockdown (Figures 5B and 5C) and the flight disability in porin mutants was rescued by Drp1 overexpression (Figure 5D).
Stress sensitivity, reduced energy stores, and CREB target gene expression in TORC mutants is rescued by neuronal TORC expression [4], indicating that CREB activity in neurons regulates energy metabolism in flies.
Mutant seedlings respond but cannot be rescued by BRs nor can the mutants be rescued by bulk sterols [ 59, 60].
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mutants were cultivated
mutants were described
mutants were confirmed
mutants were detected
mutants were obtained
mutants were recovered
mutants were discovered
mutants were set
mutants were generated
mutants were monitored
mutants were named
mutants were affected
mutants were indicated
mutants were designed
mutants was rescued
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