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However, both H432 mutants were recessive to wild-type Tdp1.
All seven mutants were recessive.
Complementation crosses indicated that all three mutants were recessive and allelic.
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Both whirlin and gelsolin mutants are recessive and thus for the double heterozygotes we would predict a wild type phenotype, which is indeed observed (Fig. 5b).
In fact, two of the new mutants are recessive lethal to females as well as males.
This suggests that each of these mutants are recessive for the somatic PGL-1 GFP-granule PGL-1 GFP-granulelikely loss-of-function alleles.
In plants heterozygous for the rlsb mutation (RLSB/rlsb-1, RLSB/rlsb-2), accumulation of LSU (and the other indicated proteins) was not affected in either leaf region; the protein profiles for these plants were identical to RLSB/RLSB, demonstrating that the insertion mutant is recessive.
The mutation is recessive, and homozygous mutants first demonstrate abnormal rostro-caudal compressions at around 72 hpf (data not shown).
The ndg-4 sa529 ndg-4 sa529 was backcrossed once to our wIII-type N2 to ensure the mutanton was recessive backcrossedrating the transheteronceoto ndg-4 mutants.
BTH-treatment of the resulting F1 progeny indicated that of 50 mutants, 35 were recessive while fifteen were dominant.
Therefore, neither screen yielded a substantial number of meiotic mutants that were recessive lethal.
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