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All combinations of Gal4, and the different GS and UAS lines and mutants were raised at 25°C.
However, our studies showed that the resistance to pathogens was compromised when Atnudt7-1 mutants were raised in MM potting soil (Fig. 2).
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To screen for mutants with a defective locomotor activity rhythm and to further characterize new activity mutants, stocks were raised at 25°C in LD 12∶12.
These eggs were grown at 20° until the appropriate day of adulthood, except for glp-1 mutants, which were raised at the restrictive temperature, 25°.
In contrast, 5-day-old rab7 mutants that were raised in constant ambient light (∼600 Lux, see 'Materials and methods') exhibit almost complete loss of synaptic function.
rict-1 mutants that were raised on the quality strain HB101 on plates were previously found to spend a greater percentage of time away from the bacterial lawn than WT animals (10.8%percentt vs. 3.1%percentt as day-one adults) (Soukas et al., 2009).
Wild-type TL, transgenic and mutant zebrafish were raised and handled in accordance with the institutional guidelines on animal usage and maintenance of Peking University.
glp-1 mutant animals were raised for 48 hr at the restrictive temperature (25°) to ablate the germline and then shifted to 20°.
White-CantonS (WCS) controls and spastin 5.75 mutant Drosophila were raised in parallel at 24°C ('untreated') or moved to 18°C during either their larval or pupal stage of development, and differences in eclosion rates determined for each condition (Fig. 1A,B).
In let-7-C and ecd mutant studies, flies were raised on standard cornmeal yeast agar-medium at 25° and fattened on wet yeast paste 1 day before dissecornmeal yeast agar-mediumted.
Wild and ktoy82 mutant embryos [6] were raised at 28.5°C and selection of embryos stages were according to [43].
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