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Exact(28)
Both putatively monomeric (L49E) and dimeric (FRPcc) mutants were produced recombinantly and purified to homogeneity under reducing conditions.
In the second phase, a novel statistical method for protein improvement was used; building on data from the first phase, only 11 targeted additional mutants were produced through site-directed mutagenesis, and the best among them achieved a >1500-fold >1500-foldt improvement over the wind type.
13C15N-IgFLNa23 15N-IgFLNa23Na23 mutants were produced in E. Coli as previously described [14].
Mutants were produced by using the QuickChange PCR mutagenesis kit (Stratagene).
Mutants were produced by site-directed mutagenesis (Quick-Change, Stratagene, La Jolla, CA) using the pHT315Ab harboring cry1Ab gene.
Site-directed mutants were produced using the Stratagene QuickChange protocol.
Similar(32)
Each of the PtmU4 site-directed mutants was produced and purified as described above.
Mutants are produced through the use of wildcards as well as by using naming parts of original pointcut and join points identified from the base code.
The mutants are produced more often at higher death rates (because of the increased total number of cell divisions).
Cycling mutants are produced by cycling wild-type cells and they grow according to the same law as the cells producing them.
Therefore, there are more cell divisions for a larger death rate, and as a consequence, more 1-hit mutants are produced.
More suggestions(15)
shifts were produced
transformants were produced
mutants were confirmed
mutants were recovered
mutants were obtained
mutants were discovered
mutants were generated
mutants were set
mutants were monitored
mutants were affected
mutants were named
mutants were indicated
mutants were expressed
mutants were designed
mutants were solved
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