Sentence examples for mutants were prepared from inspiring English sources
All mutants were prepared and evaluated along with the original FV57.
To study the effect of lysine and catalytic glutamate or aspartate on isopeptide bond formation and structural stability, alanine-substituted mutants were prepared (Supplementary Table 1).
Membranes from Sf9 or HEK293 cells expressing 6hNETBR or its mutants were prepared, and the expressed ETB receptors were quantitated as described previously62,63.
Arising from the results of theoretical calculations, three mutants were prepared by site-directed mutagenesis and characterized by fluorescence analysis.
Since there are only two Trp residues in the molecule, two single-Trp mutants were prepared to deconvolute their signals.
Three sets of labeled AK mutants were prepared, which were designed to probe the equilibrium and transient distributions of intramolecular segmental end-to-end distances.
In this report, a library of chimeric mutants was prepared from CYP2C8, CYP2C9, CYP2C18 and CYP2C19 by DNA family shuffling.
After centrifugation of cultured cells at 4000 rpm for 15 min, genomic DNA of mutants was prepared using AquaPure Genomic DNA isolation kit (Bio-Rad LaboraTokyo, Japano, Japan).
Starting from this plasmid, a total of seven single-Cys mutants was prepared: Q6C, D24C, A53C, N68C, M89C, M102C and A121C.
Purified enzyme (native and mutants) was prepared to a final molarity of 40 nM and was incubated with 0.1 M MES pH 5.5, 0.1 M KH2PO4 pH 5.5 and thymidine (Sigma) in a total reaction volume of 100 μl.
The full-length HIV-1 WT IN, the 50-212 corelytic core domain (CC), the 1-212 two-domain protein (ΔC) and the E152A point mutant were prepared as previously described [21].
