Sentence examples for mutants were picked from inspiring English sources
After two rounds of evolution, a number of positive mutants were picked out from the mutant library.
Bacillus circulans xylanase was selected as a model system, and the promising mutants were picked up by the molecular modeling.
Individual F. tularensis Tn5 mutant colonies (∼7,500 mutants) were picked and arrayed into 96-well cell culture plates in 100 µl MMH broth and were incubated at 37°C until turbid.
Single colonies of mutants were picked and transferred onto fresh TAP+P plates using a numbered grid layout.
From the M3 generation two seeds (with husk) each from 1824 independent mutants were picked and stained with phloroglucinol-HCl reagent (1% phloroglucinol in 20% HCl) for 30 minutes as per the protocol [ 41, 58] in 96 well plates.
Their ensuing progeny (F2 and F3 generation) were screened under a stereomicroscope equipped with a fluorescent light source and individual mutant animals were picked to establish mutant lines.
A total of at least two clones of each of the mutant lines were picked and screened for phenotypic characterization.
In the F2 generation, 100 mutant worms were picked and allowed to grow on 10 6-cm Petri plates close to starvation.
Single colonies of wild type Saccharomyces cerevisiae S288c BY4742 and the rpd3 YNL330C) deletion mutant (rpd3Δ:: KANMX) were picked and were inoculated into 5 mL each of liquid YPD media without and with 0.2 g/L G-418, respectively.
Although we did not perform direct follow-up analysis of the progeny of F1 animals after unc-1 targeting, we did find progeny that showed the expected uncoordinated (Unc) phenotype on the P0 plates from which mutant F1's were picked after a few generations had passed.
To recover the 402 bp gene 20 deletion mutant, ten mixed plaques were picked and re-plated and single plaques tested by PCR; at least one pure mutant was identified in seven of these, although at greatly varying frequencies (1/15, 2/15, 3/16, 10/15, 2/17, 2/24, 2/15; 19.8% average).
