Sentence examples for mutants were performed from inspiring English sources

To compare the molecular events underlying ligand affinity in the wild-type, I21V, and I16T mutant enzymes and to identify the molecular aspects related to resistance, molecular dynamics simulations of fully solvated NADH-InhA (wild-type and mutants) were performed.

YnaI and YnaI Δ2−62 were performed in asymmetrical KCl, KF and KNO3 solutions, but other mutants were performed only in asymmetrical KCl solution.

Infections with WT or ICP27 mutants were performed at a moi of 10.

The screening of potential siRNA resistant mutants were performed in our established stable shRNA-expressing cell lines according to previously described protocols [42] with some modification.

Thus, co-immunoprecipitation experiments in HeLa cells co-transfected with flag-RPB3 and myc-eEF1γ and a series of its deletion mutants were performed (fig. 2C D).

The expression and purification of recombinant quail OPN protein (OPN220) mutants were performed as described previously.

The effects of Ca2+ on circadian gene expression experiments were designed by M.J.G. and M.C.M.R. and performed by them with K.E.H., S.A., C.T.H., F.C.R. and A.N.D. Reverse genetic screening was performed by K.E.H. Analysis of cml23/cml24 mutants was performed by M.C.M.R., K.E.H., N.I.M-N., T.J.H. and H.J.J. Y-C.T. provided lines before publication and advice.

Complementation of egy1 mutants was performed to ensure the function of EGY1 (Fig. 1c).

Phenotypic analysis of these mutants was performed by their culturing on solid and liquid sucrose-THY medium.

Further phenotypic characterization of the cos1 mutants was performed.

Screening of the T21D12.3 mutants was performed as described previously [15].