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HbpR and HbpR mutants were overexpressed and purified from E. coli.
These RelB deletion mutants were overexpressed in HEK 293 cells together with HA-tagged RelA.
WT and each of these Bax mutants were overexpressed in Bax−/− MEF or lung cancer H157 cells and the proapoptotic activities were compared.
To directly verify that a phosphorylation of NPM/B23 on S4 promotes centriole duplication, NPM/B23 mutants were overexpressed in unperturbed U2OS GFP-Centrin1 cells.
Human wild-type α-Syn and the A30P, E46K and A53T mutants were overexpressed in E. coli BL21 DE3) cells using plasmid pT7-7 and purified as described previously [81] with minor modifications, as follows.
CD44 palmitoylation-impaired mutants were overexpressed in histologically normal MCF-10a cells, whereupon Triton X-100 insolubility assays confirmed reduced partitioning to raft fractions.
Similar(49)
Ectopic bristles are also observed when hyperactive Mad mutants are overexpressed in the fly wing [6].
GST-YY1, full length or various deletion mutants, was overexpressed in bacterial cells, and then bacterial lysates were used in a radioactive in vitro kinase assay with purified Plk1.
In addition, a small proportion of primary neuronal cells shows cytoplasmic localization, which is enhanced when TDP-43 mutants are overexpressed [ 13, 54, 73, 99].
This is an interesting question and points to the limits in interpreting experiments in which the kinase inactive mutants are overexpressed.
First the evidence shown in Figure 4D is not sufficient to say that "telomeres shrink" when the TEN domain mutants are overexpressed.
More suggestions(15)
mutants were discovered
mutants were set
mutants were generated
mutants were monitored
mutants were affected
mutants were named
mutants were indicated
mutants were expressed
mutants were designed
mutants were screened
mutants were solved
mutants were compared
mutants was overexpressed
mutants were tested
mutants were analyzed
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