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Homozygous mutants were only available for six different mutations.
The strains expressing the ftsZ mutants were only slightly affected by the overexpression of GFP-MinC/MinD.
Again, the highest oxygen evolving activities were recorded from HC grown cells and differences between the WT and the Δflv mutants were only minor.
However, IDH2 R172 mutants were only weakly able to pull down endogenous IDH2 compared to IDH2-WT in these cells (Fig. 1c).
While the control strains (with either pASD2 or a ΔBA4109 mutation) survived to 3 months, the BA3443, BA0672, and BA1295 mutants were only transiently detected and therefore unable to survive (Figure 9D).
gas1Δ deletion mutants were only able to grow in the presence of osmotic support (Fig. 3A), thus confirming that gas1+ is an essential gene during vegetative growth in fission yeast.
Similar(38)
Phenotype analysis of SFAR4 transgenic plants under osmotic (glucose and mannitol) stress showed that the germination rate of sfar4 knockout mutants was only 38%% after 4 days in MS medium with 5%% glucose (Fig. 3).
t-DNA insertion mutants are only available in the UTR or in the promoter regions for TPK3.
Studies on human lysozyme indicated that the structure of disease-related mutants was only slightly more disordered than those of native lysozyme.
The lifespan extension of mth mutants is also dependent upon genetic background and sex, and the reduced fecundity of mth mutants was only revealed under exposure to environmental stress [13].
Interestingly, the antioxidant rescue of lifespan for heterozygous dOpa1 mutants is only effective in the males, but not in the females in our study, which is consistent with the complex II activity.
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