The fittest, most resistant mutants were not always the fastest.
PDGFRA mutants were not responsive to their ligand, PDGFAA, and displayed a high constitutive kinase activity.
In addition, lateral line hair cells in these mutants were not sensitive to prolonged exposure to streptomycin, which is toxic to hair cells.
Importantly, the reductions in BiFC signal with the various MHC-I mutants were not due to differences in protein expression, as revealed by Western blot analysis (Fig. 1c).
Circular dichroism indicated that the structures of these mutants were not significantly perturbed relative to native PtmU4 (Supplementary Fig. 16e and Supplementary Methods).
Interestingly, the mutants were not affected by the mutation and their kinetics was very similar to the wild type strains.
It now appears, however, that these mutants were not capable of hormone-independent growth after all.
Only the double cross-over mutants could form colonies on the 7H10 agar media, the single cross-over mutants were not viable.
These data clearly indicated that the mutants were not only able to survive harsh conditions but also stable after several generations without any back mutation.
Thus, in this first stage of the study, as the mutants were not executed, there is no data about live, dead or equivalent mutants.
DRY/AAY mutants were not included in this analysis.
