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Consistent with this, the transcription levels of CaMCA1 in cmp1Δ and crz1Δ mutants were much lower than that in the wild-type cells (Fig. 4C).

Although swi3-E31 ctf18Δ and swi3-E39 ctf18Δ cells were viable, these double mutants were much more sensitive to CPT compared to either single mutant (Figure 7C).

When overexpressed, these mutants were much less effective at increasing the binucleation frequency observed in DAPI-stained HFF-hTERT cells (Figure 5B), or the frequency of cytokinesis failure viewed by live-cell DIC microscopy (Figure 5C).

Consistent with previous findings [7], FLC was highly expressed in fca mutants (Figure 3A); however, FLC was further de-repressed in clf;fca and FLC mRNA levels in the double mutants were much higher than those in fca or clf (Figure 3A).

The deletion mutants of the UBX family proteins did not display specific G1 delay at high temperature (data not shown), whereas the temperature-sensitive npl4-1 and ufd1-2 mutants were much delayed in both budding and DNA replication upon release from α-factor arrest at 38.5°C (Fig. 6A).

Overall, however, as a group small birth size mutants were much more likely to have a high %G1 DNA content.

However, the signal intensity for OsAS1 in phloem companion cells of the nodal vascular anastomoses in the gs1 2 mutants was much lower than in the wild-type rice.

Figure 1 Characterization of the asl3 mutants at 3-leaf stage: (A) WT plants (Jiahua 1) (B) asl3 mutant plants; (C) RNAi transgenic line transformed with pTCK303-dsRNAiASL3; (D) RNAi control; (E) The pigment contents in leaves at 3-leaf stage in asl3 mutants are much lower than that in WT plant.

In addition, ΔssrA and ΔsmpB mutants are much more sensitive to hygromycin than wild type strain.

For example, the total brain size of the double mutants is much smaller [10], [32].

Statistical analysis shows that the frequency of aberrant patterns in dRecQ414 mutants is much higher than in wild type control.