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ATPase activities of ΔN-TClpB mutants were measured spectrophotometrically using an ATP-regenerating system at 25 °C.
The activities of these mutants were measured using MP as substrate.
The thermostability of Bgl6 and the mutants were measured by two parameters.
Steady-state kinetic parameters for the generation of hydrocarbon products by EIZS mutants were measured as previously described.
To evaluate the temperature stabilities, the residual activities of mutants were measured after incubation at various temperatures for 1.0 hour.
To further confirm this hypothesis, the half-life of HBx and its mutants were measured as described in the Materials and Methods.
Similar(46)
The 3H4MV content in leucine analog resistant mutants was measured by high-performance liquid chromatography (HPLC).
The activity in the supernatant of all mutants was measured under the same experimental conditions (pH 4.5 and 37 °C).
The activity of wild-type hAFP and its mutants was measured by using a nanoliter osmometer (Clifton Technical Physics, Hartford, NY) as previously described [32].
The STAT5 transcriptional activity of the various mutants was measured in γ-2A cells (fibrosarcoma cells deficient in JAK2) by dual luciferase assays with the STAT reporter, pGRR5-Luc [45].
To further characterize the metabolic regimen (glycolytic or gluconeogenic) in suppressive and non suppressive contexts, the activity of the gapB promoter, an indicator of gluconeogenesis, in the WT strain and in metabolic mutants was measured.
More suggestions(15)
clones were measured
variants were measured
mutants were confirmed
mutants were recovered
mutants were obtained
mutants were discovered
mutants were generated
mutants were set
mutants were monitored
mutants were named
mutants were indicated
mutants were affected
mutants were expressed
mutants were designed
mutants were solved
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