Sentence examples for mutants were maintained from inspiring English sources
The parent strain and mutants were maintained on potato dextrose agar (HiMedia Mumbai, India) supplemented with 2 g l−l yeast extract.
The gtl-1 mutants were maintained on conventional NGM-lite plates to avoid growth defects [9].
cye-1 mutants were maintained as heterozygotes over the hT2[qIs48] balancer.
The temperature sensitive mutants were maintained in a 15°C incubator and shifted to a 26°C incubator to perform temperature upshifts for determining embryonic lethality.
RH strain T. gondii tachyzoites (wild-type and mutants) were maintained by serial passage in primary cultures of Human foreskin fibroblast (HFF) cells, as described previously [43].
C. elegans wild-type Bristol N2 animals and mutants were maintained as hermaphrodites at 20°C, grown on modified nematode growth medium (NGM) agar plates, and fed with E. coli strain OP50 as described [23].
Thus, short telomeres in tpz1-K75A mutares are maintained by impaired telomerase activity.
The constitutive activity of these mutants was maintained when associated to CTD° (H927A [ 37]), showing that an intact RNA binding site on the CTD was not required.
When tested for changes in acetylation of H4K5, the primary in vivo target of Esa1 (Clarke et al. 1999), global acetylation in nab3 mutants was maintained at wild-type levels.
This last result suggests that when rict-1 mutants are maintained in liquid, neither temperature nor food source influences their lifespan to the dramatic extent seen in plate culture.
As theTIMP-3 mutants used in the present study lack the ability to inhibit the MMPs, they were not expected to exhibit a direct effect on MMP-mediated collagen degradation, confirming that the selectivity of these N-TIMP-3 mutants was maintained ex vivo.
