Sentence examples for mutants were made from inspiring English sources
In multirepeat peptides, single-Trp mutants were made by replacing other Trp(s) with Phe.
For this purpose, four mutants were made with proline substitution (P11A) or relocation (P11A/G7P, P11A/V12P, P11A/V15P).
PHB mutants were made using site-directed mutagenesis kit (Stratagene, USA) following manufacturer's instructions.
The ura3 mutants were made by error-prone PCR mutagenesis [29].
All μ1 mutants were made using the QuickChange kit (Stratagene) and confirmed by DNA sequencing.
Additional mutants MA, MB, and MAB mutants were made based in the ALF63+256 construct.
Double mutants between these LTM strains and dunce1 mutants were made by standard procedures using chromosomal balancers.
Observation on survival of the mutants was made on 15th day after spraying.
The selection of the 30 genes from the total of 836 candidates identified with the three DNA methyltransferase mutants was made on the basis of their putative role in cell differentiation and cell division (Table S3).
Therefore a second set of mutants was made where mTNFα was changed progressively to hTNFα.
When domain-swap mutants are made between Ngn2 and NeuroD, the bHLH domain of Ngn2 is destabilising, whilst all domains of NeuroD are stabilising.
