Induced Sal-resistant mutants were isolated using procedures analogous to those used for isolation of induced Myx-resistant mutants in Mukhopadhyay et al. (2008).
The insertion mutants were isolated and inoculated into 96-well microtiter plates containing Middlebrook 7H9 medium.
Homozygous mutants were isolated, transgenic plants generated, and all plant seeds maintained in H.D. lab.
Several mutants were isolated that lose centromeric cohesins prematurely (that is during meiosis I rather than during meiosis II).
All phage mutants were isolated from a single plaque on selection S. aureus strains shown next to the corresponding mutants.
Human SNAP-23 cleaving mutants were isolated using a newly established yeast-based screening system.
(8) When E. coli umuC122 Tn5 was chemically mutagenized to block the residual gamma-radiation mutagenesis observed in umuC strains, two mutants were isolated.
Plasmids from azithromycin or erythromycin-resistant mutants were isolated, transformed into E. coli TOP10, and confirmed to be resistant to C3 cladinose macrolides.
The tetR promoter system was induced by aTet and mutants were isolated by selecting for those that were resistant to FOA.
heb3-1, heb6-1, and heb7-1 mutants were isolated by screening for mutants that exhibited severe root growth defects under high-B conditions (Fig. 1a, b).
Over 100 mutants were isolated at a frequency up to ninefold higher than that of uninfected controls.
