Sentence examples for mutants were integrated from inspiring English sources

All four shs1-ps mutants were integrated at the endogenous SHS1 locus.

The shs1-ps mutants were integrated into the genome at the SHS1 locus as previously described [60].

The lsk1 mutants were integrated at the lsk1 locus by replacement and 5-FOA selection in a lsk1::ura4 strain.

Transgenes containing ft-EGFP and point mutants were integrated into the same landing site (attP2 68A4) (Groth et al., 2004) by Genetivision (Texas, USA).

In general, mutants were integrated into the endogenous locus using PCR-based gene targeting [ 30] and replaced the wild-type allele.

All B-box mutants were integrated by standard yeast genetic techniques, involving deletion of the tDNA or ETC site by replacement with URA3, followed by transformation of site-directed mutagenized fragments and selection on 5-fluoroorotic acid (5-FOA) media.

Mutation of a conserved aspartic acid at position 469 to alanine or glycine compromises Chk1 function when the mutants are integrated as single copies, demonstrating that this domain of Chk1 is critical for function.

To facilitate genetic analyses and to eliminate the possibility of artifacts due to changes in gene copy number of histone genes expressed from plasmids, for these and subsequent experiments we generated strains in which the genes expressing the histone mutants are integrated into the genome of a common host strain at their endogenous locations (see Materials and Methods and Table 1).

Each mutant was integrated into the yeast genome at the URA3 locus in a RIF2 / rif2 Δ heterozygous diploid and verified by sequencing.

Mutant alleles were integrated by the pop-in/pop-out method using the integrative plasmid pJK210 (ura4) (Guthrie, 2004).

For microscopy (overexpression), linearized plasmids that carry rad8-GFP (under the nmt1 promoter), either wild-type or mutant derivatives, were integrated at leu1-32 locus in rad8∆ background.