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For kinetic analysis, varying concentrations of hu3S193 wild type and mutants were injected (10, 19, 38, 75 and 150 nmol/L).

Ac-AChBP (20 µg/mL) in 10 mmol/L sodium acetate pH 5.0 was immobilized to 600 response units in flow cell 2. For the collection of data, α-CTx LvIA and its mutants were injected into the flow cells in a buffer comprising 10 mmol/L HEPES pH 7.2, 150 mmol/L NaCl, and 0.005% (v/v) Tween-20 at various concentration using a 30 μL/min flow rate.

After washing away the loosely bound liposomes by a NaOH pulse, 45 µl 0.6 µM NCM or mutants were injected.

After 1 week, 5 × 10 Mcellsells retrovirally transduced with Beclin 1 (WT or S90 mutants) were injected into the upper right mammary fat pad of each mouse.

Synthetic mRNAs (500 pg) corresponding to myc-tagged wild-type XDsh and different mutants were injected into the ventral blastomeres of Xenopus embryos at the 4-cell stage.

To assess the in vivo tumorigenicity of novel KRAS variants, NIH3T3 transfectants containing empty vector or different KRAS mutants were injected subcutaneously into the dorsal flank of Balb/c nude mice.

Subsequently, 5 pg of all mutants was injected at the one-cell stage.

Plasmid DNA encoding vector control, wt Neurog1 and the Neurog1 SA179/208 mutant were injected into the lateral ventricles of dissected E15 rat brains.

10 Ba/F3 cells harbouring T315I Bcr-abl mutant were injected into the tail vein of female NOD/SCID mice (7 8 mice per group, 6 7 weeks of age, Jackson Lab).

NIH3T3 transfectants (1 × 10 cells suspended in 0.1 mL phosphate-buffered saline), containing empty vector or different KRAS mutant, were injected subcutaneously into the dorsal flank of five 5-wk-old male Balb/c nude mice.

Bone marrow cells (3 × 10) from donor mice (Vegfa fl/fl control and Vegfa fl/fl Tie2-Cre mutant) were injected into the tail vein of lethally irradiated recipient WT mice.