Sentence examples for mutants were initially from inspiring English sources

The mutants were initially identified by screening via a rapid agar plate assay and then were further examined by high-performance liquid chromatography (HPLC) and gas chromatography (GC).

Mutants were initially screened for resistance above EUCAST breakpoints63 with gradient diffusion strips following manufacturers guidelines (Liofilchem, Italy), on Mueller Hinton II agar (MHA-BD; Becton, Dickinson and Company).

Mutants were initially characterised in batch culture using glucose as carbon substrate and strains with all three modifications shown to form ethanol efficiently and rapidly at temperatures in excess of 60 °C in yields in excess of 90% of theoretical.

A total of 53 double mutants were initially screened in E. coli, then the 7 most promising were inserted into an adenovirus vector and compared in SKOV3 human ovarian carcinoma cells for sensitisation to CB1954 and two alternative prodrugs.

All deletion mutants were initially administered as an intradermal inoculum of 103 CFU.

Heterozygous mutants were initially identified by colony PCR and subsequently confirmed by Southern analysis.

The SDS-PAGE and xylanase activity profiles showed that all the mutants are initially SDS-stable and retain their tertiary structure when incubated for a short time in the presence of SDS (Fig 4D), but they all respond differently when incubated for longer time periods (Figure 5).

This unexpected change in asexual reproduction phenotype may account for the dynamics of the loss of sexual reproduction observed in our study: the decrease in the number of perithecia, reflecting the invasion of the population by female-sterile mutants, was initially rapid and then gradually slowed down until female fertility was completely lost.

The functionality of all mutants was initially established by recording whole-cell currents at a holding potential of −70 mV in response to a supersaturating concentration of AITC (200 μM), applied for 20 30 s in the presence of extracellular Ca2+ (1 mM), and by measuring the amplitudes of the currents at the peak.

When the effects of the Y254V mutant were initially examined in Mn2+, the steady-state kinetic parameter Vmax/ Km for complementary dNTP incorporation was diminished <2-fold by the presence of the mutation.

For mixed biofilms, S. gordonii Challis or the BYW1 mutant were initially incubated in THB-0.1% glucose for 24 h, the resultant biofilms washed with PBS and incubated with S. mutans GS5 cells in THG-0.5% sucrose for an additional 24 h.