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The affected genes in our mutants were induced about threefold upon carbon starvation.
The flo-1 and flo-2 mutants were induced by MNU treatment of the rice cultivar Kinmaze and their loci were localized to chromosomes 5 and 4, respectively (Satoh and Omura1981; Kaushik and Khush1991).
Preliminary data indicated the two mutants were induced in the same progenitor chromosome and dplDG82 was subsequently analyzed as above using Surveyor and WAVE analysis.
Screening of 29,595 M2 plants revealed that 23 mutants were induced by C ions with LET of 30.0 keV μm-1 at a dose of 400 Gy.
In previous studies, Y-chromosome deletion mutants were induced by X-irradiation (Lebel-Hardenack et al. 2002) and γ irradiation (Zluvova et al. 2007).
To confirm that the aberrant growth phenotypes observed in the lrx3 lrx4 and lrx3 lrx4 lrx5 mutants were induced by the T-DNA insertions, complementation experiments were performed.
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Expression of UNC-45 full-length and UNC-45 point mutants was induced with a final concentration of 100 µM IPTG for 12 h at 18 °C.
This suggests that the formation of a preorganized metal-binding site – existing in the wild-type enzyme but not in the W464 mutants – was induced by Im7.
Another possibility is that the changes in transcriptional activity and changes in nucleosome distribution in mop1 1 mutants are induced by discrete mechanisms, and not related to one another.
Lapierre and coworkers suggest that autophagy in these mutants is induced at a transcriptional level, possibly via downregulation of TOR [ 68]; however, so far no further investigations to confirm a link between the loss of Notch signaling activity and the upregulation of autophagy in glp-1 mutants have been carried out.
Expression of the 4E-BP1 mutant was induced with 1 μg/ml doxycycline treatment (Sigma-Aldrich) for 24 h.
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variants were induced
transformants were induced
transformations were induced
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mutants were cultivated
mutants were described
mutants were confirmed
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mutants were recovered
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mutants were discovered
mutants were set
mutants were generated
mutants were monitored
mutants were named
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